|
KMID : 1134820110400071025
|
|
Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 7 p.1025 ~ p.1031
|
|
|
Comparison of Selective Media for Isolation and Detection of Shigella spp. from Foods
|
|
In Ye-Won
Ha Su-Jeong
Oh Se-Wook
|
|
|
Abstract
|
|
|
|
The objective of this study was to compare the performances of conventional microbiological media used in isolation of Shigella spp. from foods. Total of six selective media, including MacConkey agar (MAC), Salmonella Shigella agar (SSA), desoxycholate citrate agar (DCA), xylose lysine desoxycholate agar (XLD), hektoen enteric agar (HEA), and CHROMagar, were tested. MAC showed almost the same colony numbers as compared to tryptic soy agar (TSA) while DCA showed significantly lower colony numbers when cultivated Shigella spp. was counted in each medium. In a food recovery test with beef, pork and shrimp, S. sonnei recovered well on CHROMagar (p＜0.05). With lettuce and cabbage, S. sonnei displayed significantly significant recovery (p＜0.05) on SSA in comparison with other selective media. Heat-injured cells recovered well on MAC and SSA. In a specificity test using Enterobacteriaceae strains, HEA was identified as having the highest specificity among the tested media. However, Morganella spp. could not be differentiated from Shigella spp. on any of the tested selective media. Shigella spp. precluded the possibility of isolation from foods by a single ‘best’ selective medium. Consequently, a combination of complementary selective media or selection of appropriate media according to cell conditions must be considered for comprehensive isolation.
|
|
|
KEYWORD
|
|
|
selective media, Shigella spp., foods, selectivity, comparison
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|